Department of Conservation says protesters on Great Barrier Island must vacate a reserve site by Thursday.

Protestors have occupied the site for two weeks in an effort to stop a pest eradication drop on nearby Rakitū Island.

Protesters fear the rat poison being dropped by air will contaminate their water and seafood resources.

Further, it's claimed DOC did not consult with all five iwi groups affiliated to the island.

In a media statement; The Department of Conservation says that they have consulted with mana whenua and have been working in partnership with the Ngāti Rehua Ngātiwai ki Aotea Trust Board to remove rats from Rakitū Island.

It's not clear exactly when DOCs plans the poison drop, but protesters fear it could happen this week.